Techniques and Protocols

Techniques used in the Taniguchi Lab include, but are not limited to:

  • Whole exome sequencing
  • RNA seq
  • Single cell RNA seq (access to 10X in lab)
  • Microbiome sequencing
  • Seahorse Extracellular metabolic flux analysis (dedicated machine in lab)
  • Hypoxia (dedicated hypoxia chamber in lab)
  • Quantitative PCR (dedicated 384-well qPCR machine in lab)
  • Western blot (Taniguchi Western Protocol Updated 2014)
  • Molecular cloning and standard biochemical techniques
  • Cloning and gene vectors using synthetic biology, CRISPR/Cas9 & Gibson cloning
  • Lentiviral gene delivery systems, often custom designed with synthetic biology
  • Adenoviral gene delivery systems (Taniguchi Lab Adenovirus Protocols)
  • Promoter assays using luciferase reporters, custom designed with synthetic biology
  • *Genetically engineered mouse models (GEMM) of pancreatic cancer, hypoxia signaling and the intestinal stem cell niche
  • *Dual recombinase (Cre and FlpO) genetic engineering
  • Small animal imaging (MRI, CT, ultrasound)
  • *Small animal targeting radiation using SARRP (Small animal Radiation Research Platform, XStrahl)
  • *Bioluminescence-guided small animal radiation
  • GEMMs +/- cytotoxic drugs and targeted RT
  • Orthotopic implantation of syngeneic tumors +/- cytotoxic drugs and RT
  • Flank tumor models +/- cytotoxic drugs and RT
  • In vivo luciferase imaging
  • Withers-Elkind clonogenic assay
  • *Ex vivo Spheroid Formation Assay (SFA) for assessing intestinal stem response to toxic insults (Publication forthcoming)

*Unique to our lab at MDACC